A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula

Polychaetes (such as Sternaspis sculata) can biotransform polycyclic aromatic hydrocarbons (PAHs) in the environment, and cytochrome P450 (CYP) enzyme is key substance this process. Here, a novel CYP gene was identified from S. sculata, marine polychaete that abundantly distributed along coasts worldwide. The full-length cDNA of new 1829 bp, encoding protein 509 amino acids. deduced acid sequence contains conserved motifs family (FxxGxxxCxG) characteristic sequences CYP4 (EVDTFMFEGHDTT), showing high similarity to CYP4V polychaetes. Therefore, might belong subfamily CYP4V, tentatively named SsCYP4V. expression levels SsCYP4V field were detected sculata sampled six sites Liaodong Peninsula. results showed relative significantly different among sampling sites; however, no positive relationship found between PAHs concentrations sediments. These findings suggested complexity field, cannot be used biomarker for monitoring PAH pollution.